LOCUS       Exported                3576 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0060_sensor_prhlRstRBS-sfGFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="spacer (B0040)"
     promoter        197..247
                     /note="P(Rhl) (I14017)"
     RBS             256..267
                     /note="RBS (B0034)"
     CDS             274..999
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1002..1118
                     /note="T1"
     terminator      1011..1097
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1125..1144
                     /note="AB179"
     primer_bind     1310..1329
                     /note="AB5"
     rep_origin      1463..2290
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1711..1713
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1714..2258)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2362..2381
                     /note="AB180"
     oriT            2392..2500
                     /note="incP origin of transfer"
     CDS             complement(2656..3471)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagaaggt tctgttaagt aactgaaccc aatgtcgtta gtgacgctta cctcttaaga
      181 ggtcactgac ctaacatcct gcatgaattg gtaggcttac gcaagaaaat ggtttgttat
      241 agtcgaatac tagagaaaga ggagaaatac tagatgagca aaggagaaga acttttcact
      301 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      361 cgtggagagg gtgaaggtga tgctacaaac ggaaaactca cccttaaatt tatttgcact
      421 actggaaaac tacctgttcc gtggccaaca cttgtcacta ctctgaccta tggtgttcaa
      481 tgcttttccc gttatccgga tcacatgaaa cggcatgact ttttcaagag tgccatgccc
      541 gaaggttatg tacaggaacg cactatatct ttcaaagatg acgggaccta caagacgcgt
      601 gctgaagtca agtttgaagg tgataccctt gttaatcgta tcgagttaaa gggtattgat
      661 tttaaagaag atggaaacat tcttggacac aaactcgagt acaactttaa ctcacacaat
      721 gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa aattcgccac
      781 aacgttgaag atggttccgt tcaactagca gaccattatc aacaaaatac tccaattggc
      841 gatggccctg tccttttacc agacaaccat tacctgtcga cacaatctgt cctttcgaaa
      901 gatcccaacg aaaagcgtga ccacatggtc cttcttgagt ttgtaactgc tgctgggatt
      961 acacatggca tggatgagct ctacaaaagg ccttaataaa ttaaaggcat caaataaaac
     1021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1081 tcctgagtag gacaaatccg ccgccctaga cagctgggcg cgccaaatac gcccggtagt
     1141 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     1201 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     1261 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     1321 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     1381 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     1441 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     1501 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     1561 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     1621 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     1681 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     1741 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     1801 ccaggcgttt cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1861 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     1921 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     1981 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     2041 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     2101 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     2161 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     2221 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
     2281 taaaatattt ctagcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2341 ggggttccgc gcacatttcc ccgaaaagtg ccacctgcta gggccggcct acggccagcc
     2401 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
     2461 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
     2521 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
     2581 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaaggaccc
     2641 ttgtccaatt aattattaga aaaattcatc cagcatcaga tgaaattgca gtttgttcat
     2701 atccggatta tcaatgccat atttctgaaa cagacgtttt tgcaggctcg ggctaaattc
     2761 gcccaggcag ttccacagaa tggccagatc ctgataacga tccgcaatgc ccacacggcc
     2821 cacatcaatg cagccaatca gtttgccttc atcgaaaatc aggttatcca ggctaaaatc
     2881 gccgtgggtc accacgctat ccgggctaaa cggcagcagt ttatgcattt ctttccacac
     2941 ctgttccacc ggccagccgt tacgttcatc atcaaaatcg ctcgcatcca ccaggccgtt
     3001 gttcatacgg ctctgcgcct gggccagacg aaacacacga tcgctgttaa acgggcagtt
     3061 gcacaccgga atgctatgca gacgacgcag aaacacggcc agcgcatcca caatgttttc
     3121 gccgctatcc ggatattctt ccagcacctg aaacgcggtt ttgcccggaa tcgcggtggt
     3181 cagcagccac gcatcatccg gggtgcgaat aaaatgttta atggtcggca gcggcataaa
     3241 ttcggtcagc cagttcagac gcaccatttc atcggtcaca tcgttcgcca cgctgccttt
     3301 gccatgtttc agaaacagtt ccggcgcatc cggtttgcca tacagacgat aaatggtcgc
     3361 gccgctctga cccacgttat cacgcgccca tttatagcca tacagatccg catccatgtt
     3421 gctgttcaga cgcggacggc tacagctcgt ttcacgctga atatggctca taacacccct
     3481 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     3541 tgcaatgtaa catcagagat tttgagacac aaattt
//
